USE OF ENZYME LINKED IMMUNOSERBENT ASSAY (ELISA) FOR THE DIAGNOSIS OF CLINICALLY SUSPECTED REFERRED HUMAN CYSTIC AND ALVEOLAR HYDATIDOSIS CASES IN NEPAL

Authors

  • D D Joshi National Zoonoses & Food Hygiene Research Centre (NZFHRC) Chagal, Kathmandu, Nepal.
  • H Joshi
  • P B Chand

DOI:

https://doi.org/10.31729/jnma.433

Abstract

The objective of the study is to diagnose suspected human cystic and alveolar hydatidosis cases in Nepal. In
this present study diagnosis of active clinical cases of cystic and alveolar hydatidosis referred to our centre
by different hospitals, nursing homes and private clinics of Kathmandu were carried out from August 1999
to March 2001 by using ELISA. Among 44 samples including 31 samples from male and 13 samples from
female. 35 (79.54%) were found positive. Regarding the age group maximum 13 samples were collected
within 6-15 years age of which 12(27.3%) were found serrologically positive. Ultrasonogram (USG) and
computerised tomography (CT scan) are the method of choice to image the hepatic and other abdominal
cysts. In this study 75% of CT scan suspected cases were found positive by ELISA. Hence these imaging
techniques must be used to detect the shape, size and location of the cyst but not as confirmative. Blood
examination results, biochemical tests and stool and urine examination have basically no relation with
hydatidosis.

Key Words: Cystic, alveolar hydatidosis, ELISA, genotype, strain.

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How to Cite

Joshi, D. D., Joshi, H., & Chand, P. B. (2004). USE OF ENZYME LINKED IMMUNOSERBENT ASSAY (ELISA) FOR THE DIAGNOSIS OF CLINICALLY SUSPECTED REFERRED HUMAN CYSTIC AND ALVEOLAR HYDATIDOSIS CASES IN NEPAL. Journal of Nepal Medical Association, 43(156). https://doi.org/10.31729/jnma.433

Issue

Vol. 43 No. 156 (2004)

Section

Original Article

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