Prevalence of Staphylococcus aureus Isolated from Clinical Samples in a Tertiary Care Hospital: A Descriptive Cross-sectional Study

  • Jyotshna Sapkota Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Sinamangal, Kathmandu Nepal
  • Manisha Sharma Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Sinamangal, Kathmandu Nepal
  • Beena Jha Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Sinamangal, Kathmandu Nepal
  • Chandra Prakash Bhatt Department of Microbiology, Kathmandu Medical College and Teaching Hospital, Sinamangal, Kathmandu Nepal
Keywords: antibiotic resistance; MRSA; Staphylococcus aureus.

Abstract

Introduction: Staphylococcus aureus is one of the commonest cause of nosocomial infections. Resistant Staphylococcus aureus strain has become a matter of concern. This study was done to find out the prevalence of Staphylococcus aureus from different clinical samples.

Methods: The descriptive cross-sectional study was carried out in the Clinical Microbiology laboratory from January 2019 to June 2019. Ethical approval was received from the Institutional Review Committee (Ref: 28122018010). Six hundred sixty-six sample size was calculated. Convenient sampling was done. Staphylococcus aureus was identified on the basis of its microscopy and morphological characteristics followed by catalase and coagulase test. Antibiotic sensitivity test of misolated pathogens was done using Muller Hinton Agar by Kirby-Bauer method. Statistical analysis was done by Excel 2018, point estimate at 95% confidence interval was calculated along with frequency and proportion for binary data.

Results: Out of the 666 bacteria isolated from clinical specimens, 133 (19.96%) were Staphylococcus aureus at 95% confidence interval (129.91-136.01%). Seventy nine (78.95%) of which is isolated from pus and wound infections. Out of 133 Staphylococcus aureus, 70.64% were Methicillin-Resistant Staphylococcus aureus.

Conclusions: This study provides valuable information regarding the high prevalence of Staphylococcus aureus from pus and wound infections. The alarming number of Methicillin-Resistant Staphylococcus aureus is worrisome finding. Antibiotics like Vancomycin and Linezolid which has not developed resistance should be cautiously used only in Methicillin-Resistant Staphylococcus aureus cases.

Published
2019-12-31
How to Cite
Sapkota, J., Sharma, M., Jha, B., & Bhatt, C. P. (2019). Prevalence of Staphylococcus aureus Isolated from Clinical Samples in a Tertiary Care Hospital: A Descriptive Cross-sectional Study . Journal of Nepal Medical Association, 57(220). https://doi.org/10.31729/jnma.4673
Section
Original Article