GeneXpert Based Confirmed Cases among Suspected Cases of Tuberculosis in a Tertiary Care Centre: A Descriptive Cross-sectional Study

Authors

  • Prabesh Pant Department of Internal Medicine, Nepalgunj Medical College and Teaching Hospital, Nepalgunj, Banke, Nepal https://orcid.org/0000-0002-1091-9307
  • Kishor Gurung Department of Microbiology, Nepalgunj Medical College and Teaching Hospital, Nepalgunj, Banke, Nepal https://orcid.org/0000-0001-5895-0242
  • Nitesh Shrestha Department of Internal Medicine, Nepalgunj Medical College and Teaching Hospital, Nepalgunj, Banke, Nepal
  • Subhechhya Basnet Department of Internal Medicine, Nepalgunj Medical College and Teaching Hospital, Nepalgunj, Banke, Nepal

DOI:

https://doi.org/10.31729/jnma.7329

Keywords:

multidrug-resistant; nucleic acid amplification test; pulmonary tuberculosis.

Abstract

Introduction: Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis mostly affecting the lungs. Due to the low sensitivity of conventional microscopy and time-consuming culture method, Nucleic acid Amplification Assay Technique is preferred because of its rapidity and sensitivity. This test also helps in finding drug resistance to Rifampicin and also curtails the transmission of disease. The study is aimed to find the prevalence of GeneXpert confirmed cases among suspected cases of tuberculosis in a tertiary care centre.

Methods: A descriptive cross-sectional study in 104 patients was conducted in a tertiary care centre from 30th Dec 2021 to 3rd Feb 2022. Ethical clearance was taken from the Institutional Review Committee (Reference number: 464/078/079). Sputum samples were collected from patients and were processed for GeneXpert under biological safety standards. GeneXpert Mycobacterium tuberculosis/rifampicin assay, sample processing, deoxyribonucleic acid extraction, and deoxyribonucleic acid amplification occurred in a fully automated cartridge-based real-time Polymerase chain reaction. A convenience sampling method was done. Collected data were coded as per variables and entered in Statistical Package for the Social Sciences version 25. Point estimate at 95% Confidence Interval was calculated along with frequency and percentage for binary data.

Results: In all 104 patients, GeneXpert detected 10 (9.62%) (3.94-15.26 at 95% Confidence Interval) positive tuberculosis cases. Out of total positive cases, there were 6 (60%) males and 4 (40%) females and there was 1 (10%) rifampicin-resistant case.

Conclusions: The prevalence of pulmonary tuberculosis among presumptive cases in our study was found to be similar to reported literature.

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Published

2022-03-11

How to Cite

Pant, P. ., Gurung, K., Shrestha, . N. ., & Basnet, S. . (2022). GeneXpert Based Confirmed Cases among Suspected Cases of Tuberculosis in a Tertiary Care Centre: A Descriptive Cross-sectional Study. Journal of Nepal Medical Association, 60(247), 250–253. https://doi.org/10.31729/jnma.7329

Issue

Vol. 60 No. 247 (2022): JNMA: Published Monthly

Section

Original Article

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Copyright (c) 2022 Prabesh Pant, Kishor Gurung

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