TY - JOUR AU - Shrestha, Subha AU - Amatya, Ritu AU - Shrestha, Raj Kumari AU - Shrestha, Rajiv PY - 2014/03/31 Y2 - 2024/03/29 TI - Frequency of Blood Culture Isolates and their Antibiogram in a Teaching Hospital JF - Journal of Nepal Medical Association JA - J Nepal Med Assoc VL - 52 IS - 193 SE - Original Article DO - 10.31729/jnma.2295 UR - http://jnma.com.np/jnma/index.php/jnma/article/view/2295 SP - 692-696 AB - <p><strong>Introduction</strong>: Bloodstream infections are associated with significant patient morbidity and mortality. Antimicrobial susceptibility patterns should guide the choice of empiric antimicrobial regimens for patients with bacteremia.</p><p><strong>Methods</strong>:  Blood sample received from the patient attending Nepal Medical College and Teaching Hospital from March 2013 – August, 2013 were subjected to for culture. Isolate identification and antimicrobial susceptibility testing was done by standard microbiological method</p><p><strong>Results</strong>:  Out of the total 2,766 blood samples, 13.3% showed bacterial growth. The percentage of neonatal septicemia was 13.3%. Staphylococcus aureus (28%) was the most common isolates followed by <em>Salmonella enterica</em> Serotype <em>Typhi</em> (22%), <em>Coagulase negative Staphylococci</em> (9.5%), <em>Salmonella enterica</em> Serotype <em>Paratyphi</em> ((7.6%) and <em>Klebsiella pneumoniae</em> (7.6%). 26.3% of the isolates of <em>Staphylococcus aureus</em> were oxacillin resistant. Most of the gram positive organisms were susceptible to amikacin and vancomycin and showed high level resistance to cefuroxime and cotrimoxazole. Out of 109 isolates of typhoid bacilli, 95.3% were resistant to nalidixic acid ,79% to ciprofloxacin and 60.5% to ofloxacin. More than 50% of the isolates of <em>Klebsiella</em> <em>pneumoniae</em> and <em>Escherichia coli</em> showed resistance to  cephalosporins and cotrimoxazole. <em>Acinetobacter spp</em> showed high resistance (more than 60%) to ceftriaxone and ofloxacin. More than 20% of the isolates of <em>Pseudomonas aeruginosa</em> were resistant to ciprofloxacin and amikacin.</p><p><strong>Conclusions</strong>: <strong> </strong>Ongoing surveillance for antimicrobial susceptibility remains essential, and will enhance efforts to identify resistance and attempt to limit its spread.</p><p><strong>Keywords</strong>: antibiotic; bacteria; blood stream infections.</p> ER -