Severe COVID-19 among Admitted COVID-19 Patients in a Tertiary Care Centre: A Descriptive Cross-sectional Study

ABSTRACT Introduction: Severe COVID-19 patients experience elevated levels of serologic indicators of inflammation which can alter blood cell lineages and cause lymphopenia. The objective of this study was to find out the prevalence of severe COVID-19 among admitted COVID-19 patients in a tertiary care centre. Methods: A descriptive cross-sectional study was conducted in a tertiary care centre from 22 June 2021 to 30 September 2021 after obtaining ethical approval from the Institutional Review Committee (Reference number: IRC-PA-146/2077-78). Confirmed COVID-19 patients by reverse transcriptase polymerase chain reaction and admitted in the COVID block during the study period were included and those who were discharged on request or referred or unavailable blood tests were excluded. A convenience sampling method was used. Point estimate and 95% Confidence Interval were calculated. Results: Among 72 admitted COVID-19 patients, 63 (87.5%) (79.86-95.14, 95% Confidence Interval) patients had severe disease. The mean neutrophil to lymphocyte ratio and mean lymphocyte to C-reactive protein ratio were 11.60±8.15 and 25.55±20.96 respectively. Conclusions: The prevalence of severe COVID-19 was higher than in other studies done in similar settings. We suggest clinical parameter-based early categorisation of COVID-19 cases to utilize limited resources during the pandemic.


INTRODUCTION
During the pandemic of COVID-19, categorising patients into severity groups allows proper utilisation of limited resources and a reduction in disease-related morbidity and mortality. 1 COVID-19 patients experience elevated levels of markers of inflammation, like C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), lactate dehydrogenase (LDH), and procalcitonin. 2,3 They have a higher level of inflammatory cytokines altering the levels of various blood cell lineages causing lymphocytopenia. These cytokines stimulate several downstream pathways, increasing the production of acute-phase reactants like CRP, and increasing the mobilization of neutrophils. This, along with stressinduced neutrophilia, likely explains the relative lymphopenia. 4 Thus, biomarkers like neutrophilto-lymphocyte ratio (NLR) and lymphocyte-to-CRP ratio (LCR) can be used in predicting the severity of COVID-19.
The objective of this study was to find out the prevalence of severe COVID-19 among admitted COVID-19 patients in a tertiary care centre. number: IRC-PA-146/2077-78). Those cases who were confirmed COVID-19 positive by reverse transcriptase polymerase chain reaction and who were admitted in the COVID block of the same hospital during the study period were included. Those cases who were discharged on request or referred or whose blood test was not available were excluded. A convenience sampling method was used. The sample size was calculated using the following formula:  7 In contrast, One study done in Iran showed severe cases in 20% only. 5 Whereas in another study done in the initial phase of the COVID-19 outbreak in China, the severe disease was seen in 15.74% of cases only (173/1099). 8 Similarly, In another study done in China, the prevalence of severe disease was 5.62% only (5/89). 9 These differences in the prevalence of severe COVID-19 can be explained by the following facts. Our study was conducted during the second wave of COVID-19 when the pandemic had the worst course affecting more people severely.This can also be explained by the fact that during the second wave, most of the patients with mild symptoms remained in home isolation and treatment while only severe cases came to the hospital. These facts could be the reasons for the higher number of severe cases in the study.

METHODS
In this study, males and females were equally (51% vs 49%) affected indicating no gender is spared from containing COVID-19 and the mean age was 54.98 years. Our results are comparable with the results of a similar study done in Iran. Here males were 57% and females were 43% (n= 70) and the mean age was 42.7 years. 5 Similar results were also noted in the study done in Turkey where 59% of males and 41% of females were involved with a mean age of 58.55 years. 6 The mean NLR found in our study for severe COVID-19 patients was 11.60 (6.51 in non-severe). This is comparable to a similar study done in India where NLR Free Full Text Articles are Available at www.jnma.com.np was more markedly raised in a severe group (10.8 vs 7.36). 10 Other studies also concluded that the higher the NLR, severe the COVID-19 and mortality. 5,11,12 When looking at the status of LCR in the severe group, it was markedly decreased (25.55 vs 62.23) indicating rising CRP and lymphopenia with the severity of COVID-19. A similar result was also demonstrated in a study done in Turkey, decreasing LCR with the disease severity. 6 This is similar to the result of a meta-analysis done in Mexico demonstrating that the LCR was decreased in severe cases. 13 These lower LCR levels in severe patients could be the result of fewer lymphocytes leading to immune dysfunction and higher CRP levels reflecting the severe systemic inflammatory response of the patients.
Considering the sampling method used in this study, the chances of selection bias are there. This study is further limited by including the admitted patients only and being a single-centre study so this result may not represent the true prevalence of the condition in other settings. We recommend further multicentric studies with large sample sizes.

CONCLUSIONS
The prevalence of severe COVID-19 in our setting was higher than in other studies done in similar settings. We suggest clinical parameter-based early categorisation of COVID-19 cases to utilise limited resources in the pandemic.